This book deals with the procedure of pretreatment, fixation, use of various moderants, sectioning with microtome and standing of cell wall, cytoplasm and nuclei, with common and specific dyes such as safranine, light and fast green, crystal violet, orange G, Cotton blue haemato xylin, carmine, giemsa, etc., of plant of all the groups included their in, and procedure of temporary and permanent preparations and squash techniques for the purpose anatomical and cytological work many versions of preparation of stain solution and procedure of staining have been described.